Strategies for improving the capability and efficiency of SARS-COV-2 detection

doi:10.3877/cma.j.issn.2095-5820.2021.03.001

Abstract

Abstract:

The global pandemic of novel coronavirus has brought great threat to the life and health of people all over the world. Currently, the prevention and treatment measures for novel coronavirus remain very limited. Nucleic acid testing is crucial for the rapid diagnosis of infected cases, the exclusion of suspected cases, and the promotion of epidemic prevention and control and clinical treatment. In this paper, the application and challenges of RT-PCR technology in novel coronavirus nucleic acid detection are reviewed, and the practical experience accumulated in our laboratory for a long time is summarized, such as increasing instruments and equipment, optimizing experimental process, strengthening information construction, and improving working conditions, to improve the ability and efficiency of nucleic acid testing, and provide a reference for nucleic acid detection of novel coronavirus in the laboratory.

Key words: Coronaviridae, Nucleic acid probe, LOD

Jiangqin Song, Meijun Chen, Weiwei Cao, Na Kang, Wenjing Lyu, Junyang Zhou. Strategies for improving the capability and efficiency of SARS-COV-2 detection[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2021, 09(03): 129-132,149.

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References 23

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设备名称	通道数	台数	工作效率(min/样本数)
全自动核酸提取仪	96	2	60/96
半自动核酸提取仪	32	3	57/32
实时荧光PCR仪	144	1	120/144
实时荧光PCR仪	96	2	107/96

设备名称	通道数	台数	工作效率(min/样本数)
全自动核酸提取仪	96	2	60/96
半自动核酸提取仪	32	3	57/32
实时荧光PCR仪	144	1	120/144
实时荧光PCR仪	96	2	107/96

岗位	人数	工作内容	工作时长(h)
A岗	2	核酸提取	4
B岗	1	试剂准备、上机扩增	8
C岗	1	标本接收、前处理	8

岗位	人数	工作内容	工作时长(h)
A岗	2	核酸提取	4
B岗	1	试剂准备、上机扩增	8
C岗	1	标本接收、前处理	8

阶段	检测人员数量（例)	提取仪检测通道数	扩增仪检测通道数	平均日检测量
阶段1	6	96	192	158
阶段2	10	96	192	857
阶段3	10	288	432	1 556

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