To explore and establish an innovative training model for the clinical microbiology interns, aiming to enhance instructional quality and improve students' practical competencies.

By innovating teaching models, integrating diversified practical teaching methods, incorporating humanistic ideological and political education and communication skills training, and systematically optimizing the internship mentoring process for clinical microbiology testing, a more scientific and standardized trainee cultivation system is established. The effectiveness of this new model was evaluated by comparing two intern cohorts: A control group trained using traditional methods and an innovation group trained under the new integrated model.

A new training model was successfully established, emphasizing innovation ability, practical skills, and professional development. The model significantly improved student engagement and operational standardization. The innovation group demonstrated superior performance in instructor evaluations, periodic assessments, theoretical scores, and practical exam results compared to the control group, with all differences being statistically significant (P＜0.05).

The proposed training model is practical and effective, systematically enhancing both the quality of internship education and students' comprehensive practical abilities. It offers a viable approach to cultivating clinical microbiology professionals who meet modern healthcare demands.