Against the backdrop of rapid development of informatization and artificial intelligence, the traditional quality assurance model for medical laboratories is increasingly unable to meet the demands of regional medical collaboration. Building a quality management system for regional networked medical laboratories characterized by standardization, intelligence and continuous improvement has become a vital direction for facilitating mutual recognition of laboratory test results, safeguarding public medical safety, and conserving social medical resources. This paper reviews the construction models, key technologies, system establishment and existing challenges of regional networked medical laboratories, so as to provide references for the high quality development of laboratory medicine.