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中华临床实验室管理电子杂志

中华临床实验室管理电子杂志 ›› 2024, Vol. 12 ›› Issue (02) : 70 -74. doi: 10.3877/cma.j.issn.2095-5820.2024.02.002

实验研究

HBV RNA用于监测慢性乙型肝炎患者临床治愈的研究和应用
李玛1, 闫凌2, 席云1, 李尚霞1, 朱冬林1,†()   
  1. 1. 510630　广东广州，中山大学附属第三医院检验科
    2. 510630　广东广州，中山大学附属第三医院综合ICU
  • 收稿日期:2023-09-09 出版日期:2024-05-28
  • 通信作者: 朱冬林
  • 基金资助:
    广东省重点领域研发计划项目(2019B020231002)

Research and application of HBV RNA detection in monitoring the clinical cure of chronic hepatitis B patients

Ma Li1, Ling Yan2, Yun Xi1, Shangxia Li1, Donglin Zhu1,†()   

  1. 1. Department of Laboratory, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou Guangdong 510630, China
    2. General ICU, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou Guangdong 510630, China
  • Received:2023-09-09 Published:2024-05-28
  • Corresponding author: Donglin Zhu
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李玛, 闫凌, 席云, 李尚霞, 朱冬林. HBV RNA用于监测慢性乙型肝炎患者临床治愈的研究和应用[J]. 中华临床实验室管理电子杂志, 2024, 12(02): 70-74.

Ma Li, Ling Yan, Yun Xi, Shangxia Li, Donglin Zhu. Research and application of HBV RNA detection in monitoring the clinical cure of chronic hepatitis B patients[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2024, 12(02): 70-74.

目的

探讨HBV RNA检测在监测慢性乙型肝炎（CHB）患者治疗中的作用。

方法

应用实时荧光定量PCR检测56例CHB患者血清中HBV DNA及HBV RNA载量，并用电化学发光法定量检测同一时期患者血清中的HBsAg。对56例CHB患者的HBV DNA和HBV RNA检测值进行Wilcoxon符号秩检验，再将这两种检测值与HBsAg定量值进行Spearman秩相关分析，分析其中的相关性。

结果

56例CHB患者血清，HBV RNA和HBsAg检测均为阳性，其中有7例HBV DNA检测为阴性。HBV DNA与HBV RNA检测值之间差异有统计学意义（P<0.05）。HBV DNA、HBV RNA载量分别与HBsAg定量进行分析，二者与HBsAg定量均无相关性（均P>0.05）。

结论

CHB患者的HBV RNA、HBsAg阳性检出率高于HBV DNA。HBsAg与HBV RNA的阳性检出率具有一致性。

关键词: 乙肝病毒RNA, 乙肝病毒DNA, 乙肝表面抗原, 慢性乙型肝炎
Objective

To investigate the role of HBV RNA detection in the treatment of chronic hepatitis B(CHB) patients.

Methods

HBV DNA and HBV RNA in serum of 56 patients with CHB were detected by real-time fluorescent quantitative PCR, and HBV surface antigen in serum of the same period was quantitatively detected by electrochemiluminescence. The Wilcoxon sign rank test was conducted on the detection values of HBV RNA and HBV DNA in 56 patients. Then, Spearman correlation analysis was made between the two values and the quantitative value of HBsAg to analyze the correlation.

Results

The serum of 56 patients with clinically diagnosis of CHB was positive for HBV RNA and HBsAg were, and 7 of them were negative for HBV DNA. The difference between HBV RNA and HBV DNA detection values was statistically significant (P<0.05). The HBV RNA and HBV DNA load were analyzed separately with HBsAg quantitation, and there was no correlation between HBV and HBsAg (all P>0.05).

Conclusions

The positive detection rate of HBV RNA and HBsAg was higher than that of HBV DNA. The positive detection of HBsAg and HBV RNA is consistent.

Key words: HBV RNA, HBV DNA, HBsAg, chronic hepatitis B
表1 CHB患者HBV DNA与HBV RNA检测、HBsAg检测情况
项目 阳性/例 阴性/例 检出率/%
HBV DNA 49 7 87.5
HBV RNA 56 0 100.0
HBV HBsAg 56 0 100.0
表2 HBV DNA和HBV RNA检测值间的比较
项目 例数 中位数/（IU/ml） Z值 P值
HBV DNA 56 313.500 4.870 <0.001
HBV RNA 56 4 430.000
表3 HBV RNA检测值与HBsAg半定量、定量值水平相关性分析/M（Q1，Q3）
项目 组别1 组别2
例数 检测值 例数 检测值
HBV RNA 28 26 700.00（744.00，15 752 500.00）IU/ml 28 983.00（225.50，12 975.00）IU/ml
HBsAg 28 5 592.50（1 405.75，7 265.00）COI a 28 639.30（225.35，999.35）IU/ml b
r值 0.193 0.132
P值 0.326 0.504
表4 HBV DNA检测值与HBsAg半定量、定量值水平相关性分析/M（Q1，Q3）
项目 组别1 组别2
例数 检测值 例数 检测值
HBV DNA 28 21 950.00（53.78，5 090 250.00）IU/ml 28 111.0（39.65，4 735.00）IU/ml
HBsAg 28 5 592.50（1 405.75，7 265.00）COI a 28 639.30（225.35，999.35）IU/ml b
r值 0.067 0.205
P值 0.732 0.296
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