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Chinese Journal of Clinical Laboratory Management(Electronic Edition) ›› 2022, Vol. 10 ›› Issue (01): 17-22. doi: 10.3877/cma.j.issn.2095-5820.2022.01.004

• Experiment Research • Previous Articles     Next Articles

Evaluation of an inactivated sample preservation solution for rapid detection of SARS-CoV-2

Li Zhao1, Roujian Lu1, Fei Ye1, Baoying Huang1, Huijuan Wang1, Wenjie Tan1,()   

  1. 1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
  • Received:2021-06-22 Online:2022-02-28 Published:2022-04-21
  • Contact: Wenjie Tan

Abstract:

Objective

To evaluate the inactivation effect of sample preservation solution containing Triton X-100 and NP40 on novel coronavirus SARS-CoV-2 and its effect on rapid nucleic acid detection.

Methods

The virus inactivation effect was assessed by measuring the change of SARS-CoV-2 virus titer before and after the treatment with sample preservation solution . The influence on the minimum detection limit and precision of SARS-CoV-2 nucleic acid detection were evaluated by elution of throat swab samples containing SARS-CoV-2 simulated virus. The stability of nucleic acid of SARS-CoV-2 in the sample preservation solution was evaluated by spiking the SARS-CoV-2 virus in the solution and storing for different time.

Results

The SARS-CoV-2 virus titer decreased at least 2.76×105 times after being treated by the sample preservation solution in 5 minutes. The sample preservation solution had no effect on the sensitivity of SARS-CoV-2 nucleic acid detection, and the CV of SARS-CoV-2 nucleic acid detection Ct value was less than 5%. The SARS-CoV-2 virus can be stored in the sample preservation solution for at least 4 days at 25 ℃ or 2~8 ℃.

Conclusion

The sample storage solution containing surfactants Triton X-100 and NP40 can effectively inactivate virus, and the virus nucleic acid detection is not affected after inactivation, which is suitable for rapid nucleic acid detection.

Key words: Coronaviridae, Organ preservation solutions, Surface-active agents, Nucleic acids

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