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中华临床实验室管理电子杂志 ›› 2014, Vol. 02 ›› Issue (01) : 4 -10. doi: 10.3877/cma.j.issn.2095-5820.2014.01.002

综述

临床微生物实验室自动化现状与进展
杨启文1, 徐英春1,()   
  1. 1.100730 中国医学科学院 北京协和医学院 北京协和医院检验科
  • 收稿日期:2014-03-03 出版日期:2014-02-28
  • 通信作者: 徐英春
  • 基金资助:
    国家自然科学基金资助项目(H1908, 81101287)

Present situation and progress of automation in clinical microbiology laboratory

Qiwen Yang1, Yingchun Xu1,()   

  1. 1.Department of Laboratory Medicine, Peking Union Medical College/Hospital, Chinese Academy of Medinal Sciences, Beijing 100730, China
  • Received:2014-03-03 Published:2014-02-28
  • Corresponding author: Yingchun Xu
引用本文:

杨启文, 徐英春. 临床微生物实验室自动化现状与进展[J/OL]. 中华临床实验室管理电子杂志, 2014, 02(01): 4-10.

Qiwen Yang, Yingchun Xu. Present situation and progress of automation in clinical microbiology laboratory[J/OL]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2014, 02(01): 4-10.

随着微生物检验技术的发展,特别是基质辅助激光解析电离-飞行时间质谱技术以及微生物标本液体转运技术的运用,使微生物实验室自动化愈加可行。人们期盼的成功的自动化系统设计灵活,能将实验室人员从一些技术要求较低的、能由仪器完成的工作中解脱出来,从而提高标本处理效率,并减少资源浪费。目前,研发和使用的微生物实验室自动化系统可分为三大部分,第一部分为标本处理系统,第二部分为能提供全微生物实验室自动化解决方案的系统,包括为达到不同级别自动化程度的各种模块,第三部分为各种微生物自动化鉴定和药敏系统。现在,临床微生物检验流程已进入革新时代,虽然大量的评估工作还有待完成,但微生物自动化系统必将对临床微生物实验室工作水平的提高与技术发展产生深远的影响。

The automation of clinical microbiology laboratory has become more and more available with the development of technology of clinical microbiology, especially the application of the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and liquid-based microbiology specimen transport system. A successful automation system requires flexibility in the design, which can liberate the personnel of laboratory from some tasks that can be done by the instruments, thereby improving the efficiency of specimen process and reducing the waste of resources. Currently, there are three parts of automation system applied in the clinical microbiology laboratory. The first one is the specimen processor system. The second one is the solution system offering full microbiology laboratory automation, including various modules for attaining diverse automation degree. The third one is the automatic bacteria identification/antimicrobial susceptibility testing system. Nowadays, the detection process of clinical microbiology has entered the era of innovation. Although a lot of assessment works remains to be done, the microbe automatic system will have a profound impact on improving the work standard and technology of clinical microbiology laboratories.

表1 各种自动化标本处理仪的比较
图1 Kiestra TLA系统的构成图 注:图由BD公司提供,BD KiestraTM可自动对液态及非液态生物标本接种并划线分离菌株,同时整合了双向传输轨道、智能孵育系统、数字平皿影像系统,配备人工工作台,并与BD-Bruker MALDI Biotyper快速微生物鉴定系统/Phoenix全自动药敏系统自动连接
图2 FMLA系统的构成图 注:图由生物梅里埃公司提供,标本通过Previ ISOLA接种后,实验室人员将平皿放至传递仓,传送轨道自动将平皿送至指定孵育条件的孵育箱中孵育和动态高清拍照
图3 Kiestra TLA数字化平皿阅读系统 注:Kiestra TLA图片由BD公司提供,数字化影像工作站可自动获取/分析/存储培养箱中的微生物高清影像,可测量抑菌圈大小,分析并添加注解;还可在同一屏幕显示同一患者的所有微生物培养结果,以便于分析诊断
图4 FMLA数字化平皿阅读系统 注: FMLA图由生物梅里埃公司提供,数字化影像工作站可自动获取/分析/存储培养箱中的微生物高清影像,并添加注解
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